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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   VDAC1 Antibody (Center)   

VDAC1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - VDAC1 Antibody (Center) AP6627c
    Western blot analysis of VDAC1 Antibody (Center) (Cat. #AP6627c) in HL-60, Y79 cell line lysates (35ug/lane). VDAC1 (arrow) was detected using the purified Pab.
  • IHC-P - VDAC1 Antibody (Center) AP6627c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with VDAC1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - VDAC1 Antibody (Center) AP6627c
    VDAC1 Antibody (Center) (Cat. #AP6627c) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - VDAC1 Antibody (Center) AP6627c
    Western blot analysis of VDAC1 (arrow) using rabbit polyclonal VDAC1 Antibody (Center) (Cat. #AP6627c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VDAC1 gene.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P21796
Other Accession Q9Z2L0, Q9TT15, Q60932, P45879
Reactivity Human
Predicted Bovine, Mouse, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 30773 Da
Antigen Region 95-124 aa
Additional Information
Gene ID 7416
Other Names Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC1, VDAC
Target/Specificity This VDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-124 amino acids from the Central region of human VDAC1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVDAC1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VDAC1
Synonyms VDAC
Function Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756).
Cellular Location Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Membrane raft; Multi-pass membrane protein
Tissue Location Heart, liver and skeletal muscle.
Research Areas
Citations (0)

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Background

VDAC1 forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. The protein may participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

References

Shoshan-Barmatz,V., Biochim. Biophys. Acta 1787 (5), 421-430 (2009)
Hiller,S., Science 321 (5893), 1206-1210 (2008)

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$ 295.00
$ 99.00
Cat# AP6627c
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(40 western blots)
Availability: In Stock
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