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GLS2 Antibody (C-term E513)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - GLS2 Antibody (C-term E513) AP6650D
    All lanes : Anti-GLS2 Antibody (C-term E513) at 1:1000 dilution Lane 1: Human brain lysate Lane 2: Mouse liver lysate Lane 3: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - GLS2 Antibody (C-term E513) AP6650D
    Immunohistochemical analysis of paraffin-embedded H.brain section using GLS2 Antibody (C-term E513)(Cat#AP6650d). AP6650d was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • WB - GLS2 Antibody (C-term E513) AP6650D
    Western blot analysis of lysate from human brain tissue lysate, using GLS2 Antibody (C-term E513)(Cat. #AP6650d). AP6650d was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - GLS2 Antibody (C-term E513) AP6650D
    GLS2 Antibody (C-term E513) (Cat. #AP6650d) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GLS2 antibody detected the GLS2 protein (arrow).
  • IHC-P - GLS2 Antibody (C-term E513) AP6650D
    GLS2 antibody(C-term E513) (Cat. #AP6650d) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS2 antibody(C-term E513) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9UI32
Other Accession P28492, Q571F8, NP_037399.2
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 66323 Da
Antigen Region 498-524 aa
Additional Information
Gene ID 27165
Other Names Glutaminase liver isoform, mitochondrial, GLS, L-glutaminase, L-glutamine amidohydrolase, GLS2, GA
Target/Specificity This GLS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 498-524 amino acids of human GLS2.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGLS2 Antibody (C-term E513) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GLS2
Synonyms GA
Function Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Cellular Location Mitochondrion.
Tissue Location Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas.
Research Areas
Citations ( 0 )

Background

The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. This protein is functionally similar to the kidney glutaminase but is a little smaller in size. Originally thought to be liver-specific, this protein has been found in other tissues as well. At least one transcribed pseudogene has been found for this gene. [provided by RefSeq].

References

Hu, W., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7455-7460(2010)
Suzuki, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7461-7466(2010)
Szeliga, M., et al. Glia 57(9):1014-1023(2009)
Tian, C., et al. J. Neurochem. 105(3):994-1005(2008)
Maeshima, H., et al. Prog. Neuropsychopharmacol. Biol. Psychiatry 31(7):1410-1418(2007)

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$ 295.00
$ 99.00
Cat# AP6650D
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(40 western blots)
Availability: In Stock
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