|Application ||WB, IHC-P, E|
|Other Accession||Q8BGC0, Q5H919|
|Calculated MW||85853 Da|
|Antigen Region||1-30 aa|
|Other Names||HIV Tat-specific factor 1, Tat-SF1, HTATSF1|
|Target/Specificity||This HTATSF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HTATSF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HTATSF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
HTATSF1 functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation.
Miller,H.B., PLoS ONE 4 (5), E5710 (2009)
Remoli,A.L., Biochem. J. 396 (2), 371-380 (2006)
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1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose