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ZWINT Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6686c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ZWINT Antibody (Center) - Product info | |
| Application | WB, IHC, FC
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| Primary Accession | O95229 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 31293 Da |
ZWINT Antibody (Center) - Additional info | |
| Gene ID 11130 | |
| Other Names ZWINT; ZW10 interactor; ZW10-interacting protein 1 | |
| Target/Specificity This ZWINT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66~96 amino acids from the Center region of human ZWINT. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ZWINT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ZWINT Antibody (Center) - Protein Information | |
| Name ZWINT | |
| Function Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase | |
| Cellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase | |
ZWINT Antibody (Center) - Related products
AP6686b: ZWINT Antibody (C-term)
AP6686c: ZWINT Antibody (Center)
RI15858: ZWINT predesign siRNA
DC05033: Human ZWINT cDNA Clone
BP6686b: ZWINT Antibody (C-term) Blocking Peptide
BP6686c: ZWINT Antibody (Center) Blocking Peptide
ZWINT Antibody (Center) - Application data
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Western blot analysis of ZWINT antibody (Center) (Cat. #AP6686c) in mouse cerebellum tissue lysates (35ug/lane). ZWINT (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with ZWINT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of widr cells using ZWINT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of ZWINT (arrow) using rabbit polyclonal ZWINT Antibody (Center) (Cat. #AP6686c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ZWINT gene.
ZWINT Antibody (Center) - Research Areas
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BACKGROUND
ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.
REFERENCES
Famulski,J.K., J. Cell Biol. 180 (3), 507-520 (2008) Kops,G.J., J. Cell Biol. 169 (1), 49-60 (2005) Wang,H., J. Biol. Chem. 279 (52), 54590-54598 (2004)
