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PAX4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PAX4 Antibody (Center) AP6690C
    All lanes : Anti-PAX4 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: rat liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PAX4 Antibody (Center) AP6690C
    Western blot analysis of PAX4 antibody (Center) (RB20216) in CEM cell line lysates (35ug/lane). PAX4 (arrow) was detected using the purified Pab.
  • IHC-P - PAX4 Antibody (Center) AP6690C
    Formalin-fixed and paraffin-embedded human brain tissue reacted with PAX4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - PAX4 Antibody (Center) AP6690C
    Flow cytometric analysis of widr cells using PAX4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43316
Other Accession P32115
Reactivity Human, Rat
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 37833 Da
Antigen Region 171-200 aa
Additional Information
Gene ID 5078
Other Names Paired box protein Pax-4, PAX4
Target/Specificity This PAX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the Central region of human PAX4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAX4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAX4
Function Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity.
Cellular Location Nucleus.
Research Areas
Citations (0)

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PAX4 is a member of the paired box (PAX) family of transcription factors. These proteins play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells.


Hata,S., Int. J. Oncol. 33 (5), 1065-1071 (2008)
Zhang,Y., Diabetes Res. Clin. Pract. 81 (3), 365-369 (2008)

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$ 295.00
$ 99.00
Cat# AP6690C
(40 western blots)
Availability: In Stock
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