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ADH1B Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP6738c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP6738c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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ADH1B Antibody (Center) - Product info | |
| Application | WB, IHC, FC, IF
|
| Primary Accession | P00325 |
| Other Accession | P06757, P00329, P23991, P00326, P07327 |
| Reactivity | Human |
| Predicted | Chicken, Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 39855 Da |
ADH1B Antibody (Center) - Additional info | |
| Gene ID 125 | |
| Other Names ADH1B; ADH2; Alcohol dehydrogenase 1B; Alcohol dehydrogenase subunit beta | |
| Target/Specificity This ADH1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~245 amino acids from the Center region of human ADH1B. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ADH1B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ADH1B Antibody (Center) - Protein Information | |
| Name ADH1B | |
| Synonyms ADH2 | |
| Cellular Location Cytoplasm. | |
ADH1B Antibody (Center) - Related products
AP6738c: ADH1B Antibody (Center)
RI10139: ADH1B predesign siRNA
ADH1B Antibody (Center) - Application data
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Western blot analysis of ADH1B Antibody (Center) (Cat. #AP6738c) in HepG2 cell line lysates (35ug/lane). ADH1B (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADH1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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ADH1B Antibody (Center) (Cat.#AP6738c) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Confocal immunofluorescent analysis of ADH1B Antibody (Center)(Cat#AP6738c) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ADH1B Antibody (Center) - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The protein is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism.
REFERENCES
Alcohol intake, Am. J. Gastroenterol. 104 (9), 2182-2188 (2009) Nishimura,F.T., Nihon Arukoru Yakubutsu Igakkai Zasshi 44 (3), 139-155 (2009)