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ADH1B Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP6738c 0.1 mg 400 ul In Stock $ 255.00 Add to cart
AP6738c-ev20 20 ug 100 ul In Stock $ 95.00 Add to cart
  • Specification
  • Citiations : 0
  • Reviews
  • Protocols
  • Backgrounds

ADH1B Antibody (Center) - Product info

ApplicationWB, IHC, FC, IF
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionP00325
Other AccessionP06757, P00329, P23991, P00326, P07327
ReactivityHuman
PredictedChicken, Mouse, Rat
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW39855 Da

ADH1B Antibody (Center) - Additional info

Gene ID 125
Other Names
ADH1B; ADH2; Alcohol dehydrogenase 1B; Alcohol dehydrogenase subunit beta
Target/Specificity
This ADH1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~245 amino acids from the Center region of human ADH1B.
Dilution
WB~~1:50~100
IHC~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
ADH1B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

ADH1B Antibody (Center) - Protein Information

Name ADH1B
Synonyms ADH2
Cellular Location
Cytoplasm.

ADH1B Antibody (Center) - Related products

AP6738c: ADH1B Antibody (Center)

RI10139: ADH1B predesign siRNA

BP6738c: ADH1B Antibody (Center) Blocking Peptide

AJ1023b: Aldh1A1 Antibody

AJ1023c: Aldh1A1 Antibody

ADH1B Antibody (Center) - Application data

  • Western blot analysis of ADH1B Antibody (Center) (Cat. #AP6738c) in HepG2 cell line lysates (35ug/lane). ADH1B (arrow) was detected using the purified Pab.

  • Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADH1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • ADH1B Antibody (Center) (Cat.#AP6738c) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Confocal immunofluorescent analysis of ADH1B Antibody (Center)(Cat#AP6738c) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

ADH1B Antibody (Center) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

The protein is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism.

REFERENCES

Alcohol intake, Am. J. Gastroenterol. 104 (9), 2182-2188 (2009) Nishimura,F.T., Nihon Arukoru Yakubutsu Igakkai Zasshi 44 (3), 139-155 (2009)