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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ADH1B Antibody (Center)   

ADH1B Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ADH1B Antibody (Center) AP6738C
    All lanes : Anti-ADH1B Antibody (Center) at 1:2000 dilution Lane 1: human liver lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ADH1B Antibody (Center) AP6738C
    Western blot analysis of ADH1B Antibody (Center) (Cat. #AP6738c) in HepG2 cell line lysates (35ug/lane). ADH1B (arrow) was detected using the purified Pab.
  • IHC-P - ADH1B Antibody (Center) AP6738C
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADH1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ADH1B Antibody (Center) AP6738C
    ADH1B Antibody (Center) (Cat.#AP6738c) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - ADH1B Antibody (Center) AP6738C
    Confocal immunofluorescent analysis of ADH1B Antibody (Center)(Cat#AP6738c) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00325
Other Accession P06757, P00329, P23991, P00326, P07327
Reactivity Human
Predicted Chicken, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39855 Da
Antigen Region 209-237 aa
Additional Information
Gene ID 125
Other Names Alcohol dehydrogenase 1B, Alcohol dehydrogenase subunit beta, ADH1B, ADH2
Target/Specificity This ADH1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-237 amino acids from the Central region of human ADH1B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADH1B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADH1B
Synonyms ADH2
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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The protein is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism.


Alcohol intake, Am. J. Gastroenterol. 104 (9), 2182-2188 (2009) Nishimura,F.T., Nihon Arukoru Yakubutsu Igakkai Zasshi 44 (3), 139-155 (2009)

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$ 295.00
$ 99.00
Cat# AP6738C
(40 western blots)
Availability: In Stock
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