|Application ||WB, IHC-P, FC, E|
|Other Accession||P36378, P16975, P36233, P20112, P07214, P36377, P13213|
|Predicted||Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus|
|Calculated MW||34632 Da|
|Antigen Region||224-251 aa|
|Other Names||SPARC, Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, SPARC, ON|
|Target/Specificity||This SPARC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPARC Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.|
|Cellular Location||Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).
Said,N., Oncogene 28 (39), 3487-3498 (2009)
Maloney,S.C., Anticancer Res. 29 (8), 3059-3064 (2009)
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