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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SPARC Antibody (C-term)   

SPARC Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SPARC Antibody (C-term) AP6743B
    Western blot analysis of SPARC Antibody (C-term) (Cat. #AP6743b) in Y79 cell line lysates (35ug/lane). SPARC (arrow) was detected using the purified Pab.
  • WB - SPARC Antibody (C-term) AP6743B
    Western blot analysis of SPARC (arrow) using rabbit polyclonal SPARC Antibody (C-term) (Cat. #AP6743b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.
  • IHC-P - SPARC Antibody (C-term) AP6743B
    Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - SPARC Antibody (C-term) AP6743B
    Flow cytometric analysis of widr cells using SPARC Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09486
Other Accession P36378, P16975, P36233, P20112, P07214, P36377, P13213
Reactivity Human
Predicted Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34632 Da
Antigen Region 224-251 aa
Additional Information
Gene ID 6678
Other Names SPARC, Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, SPARC, ON
Target/Specificity This SPARC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPARC Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ON
Function Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Cellular Location Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane
Research Areas
Citations (0)

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Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).


Said,N., Oncogene 28 (39), 3487-3498 (2009)
Maloney,S.C., Anticancer Res. 29 (8), 3059-3064 (2009)

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Cat# AP6743B
(40 western blots)
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