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Villin-1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Villin-1 Antibody (N-term) AP6774a
    All lanes : Anti-Villin-1 Antibody (N-term) at 1:8000 dilution Lane 1: HT-29 whole cell lysate Lane 2: SW480 whole cell lysate Lane 3: Mouse colon lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Villin-1 Antibody (N-term) AP6774a
    All lanes : Anti-Villin-1 Antibody (N-term) at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: COLO 205 whole cell lysate Lane 4: Mouse colon lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Villin-1 Antibody (N-term) AP6774a
    All lanes : Anti-Villin-1 Antibody (N-term) at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: COLO 205 whole cell lysate Lane 4: Mouse kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Villin-1 Antibody (N-term) AP6774a
    All lanes : Anti-Villin-1 Antibody (N-term) at 1:1000 dilution Lane 1: Caco2 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - Villin-1 Antibody (N-term) AP6774a
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with Villin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - Villin-1 Antibody (N-term) AP6774a
    Flow cytometric analysis of WiDr cells using Villin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P09327
Other Accession Q29261, Q62468, Q3SZP7
Reactivity Human, Mouse
Predicted Bovine, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 92695 Da
Antigen Region 180-207 aa
Additional Information
Gene ID 7429
Other Names Villin-1, VIL1, VIL
Target/Specificity This Villin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-207 amino acids from the N-terminal region of human Villin-1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVillin-1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIL1
Synonyms VIL
Function Epithelial cell-specific Ca(2+)-regulated actin- modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
Cellular Location Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip. Cell projection, filopodium. Note=Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment.
Tissue Location Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level).
Research Areas
Citations (0)

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Background

Villin-1 is a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments.

References

Yamamichi,N., et.al., Exp. Cell Res. 315 (10), 1779-1789 (2009)

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$ 295.00
$ 99.00
Cat# AP6774a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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