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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ACADM Antibody (Center)   

ACADM Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ACADM Antibody (Center) AP6827c
    Western blot analysis of ACADM Antibody (Center) (Cat. #AP6827c) in mouse heart tissue lysates (35ug/lane). ACADM (arrow) was detected using the purified Pab.
  • IHC-P - ACADM Antibody (Center) AP6827c
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ACADM Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ACADM Antibody (Center) AP6827c
    Flow cytometric analysis of widr cells using ACADM Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - ACADM Antibody (Center) AP6827c
    Confocal immunofluorescent analysis of ACADM Antibody (Center)(Cat#AP6827c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, IF, E
Primary Accession P11310
Other Accession Q8HXY8
Reactivity Human, Mouse
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46588 Da
Antigen Region 189-217 aa
Additional Information
Gene ID 34
Other Names Medium-chain specific acyl-CoA dehydrogenase, mitochondrial, MCAD, ACADM
Target/Specificity This ACADM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the Central region of human ACADM.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACADM Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACADM
Function Acyl-CoA dehydrogenase specific for acyl chain lengths of 4 to 16 that catalyzes the initial step of fatty acid beta- oxidation. Utilizes the electron transfer flavoprotein (ETF) as an electron acceptor to transfer electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)

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Background

ACADM is the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway.

References

Ferreira,A.C., et.al., Genet. Mol. Res. 8 (2), 487-493 (2009)

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$ 295.00
$ 99.00
Cat# AP6827c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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