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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   ALAD Antibody (C-term)   

ALAD Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ALAD Antibody (C-term) AP6828b
    Western blot analysis of ALAD Antibody (C-term) (Cat. #AP6828b) in Jurkat cell line lysates (35ug/lane). ALAD (arrow) was detected using the purified Pab.
  • IHC-P - ALAD Antibody (C-term) AP6828b
    Formalin-fixed and paraffin-embedded human brain tissue reacted with ALAD Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ALAD Antibody (C-term) AP6828b
    ALAD Antibody (C-term) (Cat. #AP6828b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13716
Other Accession Q60HH9
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36295 Da
Antigen Region 244-272 aa
Additional Information
Gene ID 210
Other Names Delta-aminolevulinic acid dehydratase, ALADH, Porphobilinogen synthase, ALAD
Target/Specificity This ALAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-272 amino acids from the C-terminal region of human ALAD.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALAD Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.
Research Areas
Citations (0)

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The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.


Guey,L.T.,, Eur. Urol. (2009)

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$ 295.00
$ 99.00
Cat# AP6828b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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