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ASS Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ASS Antibody (Center) AP6829c
    All lanes : Anti-ASS Antibody (Center) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: PC-12 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ASS Antibody (Center) AP6829c
    All lanes : Anti-ASS Antibody (Center) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MOLT-4 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ASS Antibody (Center) AP6829c
    All lanes : Anti-ASS Antibody (Center) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MOLT-4 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ASS Antibody (Center) AP6829c
    All lanes : Anti-ASS Antibody (Center) at 1:2000 dilution Lane 1: MOLT-4 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ASS Antibody (Center) AP6829c
    Western blot analysis of ASS Antibody (Center) (Cat. #AP6829c) in Hela cell line lysates (35ug/lane). ASS (arrow) was detected using the purified Pab.
  • IHC-P - ASS Antibody (Center) AP6829c
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ASS Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ASS Antibody (Center) AP6829c
    Flow cytometric analysis of widr cells using ASS Antibody (Center)(bottom histogram) compared to a negative control (PBS alone)(top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - ASS Antibody (Center) AP6829c
    Confocal immunofluorescent analysis of ASS Antibody (Center)(Cat#AP6829c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession P00966
Other Accession P09034, P16460
Reactivity Human, Mouse, Rat
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46530 Da
Antigen Region 192-221 aa
Additional Information
Gene ID 445
Other Names Argininosuccinate synthase, Citrulline--aspartate ligase, ASS1, ASS
Target/Specificity This ASS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-221 amino acids from the Central region of human ASS.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsASS Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ASS1
Synonyms ASS
Function Is indirectly involved in the control of blood pressure.
Research Areas
Citations (0)

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Background

ASS catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase.

References

Engel,K., et.al., Hum. Mutat. 30 (3), 300-307 (2009)

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$ 295.00
$ 99.00
Cat# AP6829c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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