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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   TCPR1 Antibody (N-term)   

TCPR1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - TCPR1 Antibody (N-term) AP6845a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with TCPR1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • WB - TCPR1 Antibody (N-term) AP6845a
    Western blot analysis of TCPR1 Antibody (N-term) (Cat. #AP6845a) in A375 cell line lysates (35ug/lane). TCPR1 (arrow) was detected using the purified Pab.
  • FC - TCPR1 Antibody (N-term) AP6845a
    TCPR1 Antibody (N-term) (Cat. #AP6845a) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q7Z6L1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 129696 Da
Antigen Region 162-189 aa
Additional Information
Gene ID 25851
Other Names Tectonin beta-propeller repeat-containing protein 1, TECPR1, KIAA1358
Target/Specificity This PALA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-189 amino acids from the N-terminal region of human TCPR1.
Dilution IHC-P~~1:50~100
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTCPR1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TECPR1
Synonyms KIAA1358
Function Tethering factor involved in autophagy. Involved in autophagosome maturation by promoting the autophagosome fusion with lysosomes: acts by associating with both the ATG5-ATG12 conjugate and phosphatidylinositol-3-phosphate (PtdIns(3)P) present at the surface of autophagosomes. Also involved in selective autophagy against bacterial pathogens, by being required for phagophore/preautophagosomal structure biogenesis and maturation.
Cellular Location Cytoplasmic vesicle, autophagosome membrane. Lysosome membrane. Note=Localizes to Lysosome membranes, and binds PtdIns(3)P at the surface of autophagosome. Localizes to autolysosomes, a vesicle formed by the fusion between autophagosomes and lysosomes
Research Areas
Citations (0)

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References

Scherer,S.W., et.al., Science 300 (5620), 767-772 (2003)

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$ 295.00
$ 99.00
Cat# AP6845a
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(40 western blots)
Availability: In Stock
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