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PAX8 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PAX8 Antibody (Center) AP6874C
    All lanes : Anti-PAX8 Antibody (Center) at 1:1000 dilution Lane 1: SK-OV-3 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PAX8 Antibody (Center) AP6874C
    Western blot analysis of PAX8 Antibody (Center) (Cat. #AP6874c) in HL-60 cell line lysates (35ug/lane). PAX8 (arrow) was detected using the purified Pab.
  • IHC-P - PAX8 Antibody (Center) AP6874C
    Formalin-fixed and paraffin-embedded mouse kidney reacted with PAX8 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - PAX8 Antibody (Center) AP6874C
    Flow cytometric analysis of 293 cells using PAX8 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q06710
Other Accession Q00288
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48218 Da
Antigen Region 285-313 aa
Additional Information
Gene ID 7849
Other Names Paired box protein Pax-8, PAX8
Target/Specificity This PAX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-313 amino acids from the Central region of human PAX8.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAX8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAX8
Function Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
Cellular Location Nucleus.
Tissue Location Expressed in the excretory system, thyroid gland and Wilms tumors
Research Areas
Citations (0)

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PAX8 is a member of the paired box (PAX) family of transcription factors. Members of this family contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes.


Ramos,H.E.,, Thyroid 19 (1), 61-68 (2009)

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$ 295.00
$ 99.00
Cat# AP6874C
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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