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GSS Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - GSS Antibody (C-term) AP6895b
    Immunohistochemical analysis of paraffin-embedded H. stomach section using GSS Antibody (C-term)(Cat#AP6895b). AP6895b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IHC-P - GSS Antibody (C-term) AP6895b
    Immunohistochemical analysis of paraffin-embedded H. stomach section using GSS Antibody (C-term)(Cat#AP6895b). AP6895b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IF - GSS Antibody (C-term) AP6895b
    Fluorescent image of U-87 MG cells stained with GSS Antibody (C-term)(Cat#AP6895b). AP6895b was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • WB - GSS Antibody (C-term) AP6895b
    GSS Antibody (C-term) (Cat.# AP6895b) western blot analysis in Hela,293T,MDA-MB-453 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the GSS antibody detected the GSS protein (arrow).
  • WB - GSS Antibody (C-term) AP6895b
    GSS Antibody (C-term) (Cat. #AP6895b) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the GSS antibody detected the GSS protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P, E
Primary Accession P48637
Other Accession P46413, P51855, Q8HXX5, Q5EAC2
Reactivity Human
Predicted Bovine, Monkey, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52385 Da
Antigen Region 372-400 aa
Additional Information
Gene ID 2937
Other Names Glutathione synthetase, GSH synthetase, GSH-S, Glutathione synthase, GSS
Target/Specificity This GSS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-400 amino acids from the C-terminal region of human GSS.
Dilution IHC-P~~1:25
IF~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSS
Research Areas
Citations ( 0 )

Background

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. GSS functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione.

References

Starr,J.M., et.al., Mech. Ageing Dev. 129 (12), 745-751 (2008)

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$ 295.00
$ 99.00
Cat# AP6895b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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