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GALE Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GALE Antibody (Center) AP6902c
    Western blot analysis of GALE Antibody (Center) (Cat. #AP6902c) in A375 cell line lysates (35ug/lane). GALE (arrow) was detected using the purified Pab.
  • IHC-P - GALE Antibody (Center) AP6902c
    Formalin-fixed and paraffin-embedded human skin tissue reacted with GALE Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - GALE Antibody (Center) AP6902c
    GALE Antibody (Center) (Cat.#AP6902c) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - GALE Antibody (Center) AP6902c
    Confocal immunofluorescent analysis of GALE Antibody (Center)(Cat#AP6902c) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14376
Other Accession Q8R059, Q3T105
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38282 Da
Antigen Region 142-171 aa
Additional Information
Gene ID 2582
Other Names UDP-glucose 4-epimerase, Galactowaldenase, UDP-N-acetylgalactosamine 4-epimerase, UDP-GalNAc 4-epimerase, UDP-N-acetylglucosamine 4-epimerase, UDP-GlcNAc 4-epimerase, UDP-galactose 4-epimerase, GALE (HGNC:4116)
Target/Specificity This GALE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-171 amino acids from the Central region of human GALE.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGALE Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GALE (HGNC:4116)
Function Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N- acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6- phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP- GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.
Research Areas
Citations (0)

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UDP-galactose-4-epimerase catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.


Openo,K.K.,, Am. J. Hum. Genet. 78 (1), 89-102 (2006)

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$ 295.00
$ 99.00
Cat# AP6902c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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