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PAX6 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - PAX6 Antibody (Center) AP6929c
    PAX6 Antibody (Center) (Cat.# AP6929c) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PAX6 antibody detected the PAX6 protein (arrow).
  • IHC-P - PAX6 Antibody (Center) AP6929c
    Formalin-fixed and paraffin-embedded human brain tissue with PAX6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - PAX6 Antibody (Center) AP6929c
    Confocal immunofluorescent analysis of PAX6 Antibody (Center)(Cat#AP6929c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
  • FC - PAX6 Antibody (Center) AP6929c
    Flow cytometric analysis of hela cells using PAX6 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P26367
Other Accession P55864, P63016, P63015, Q1LZF1
Reactivity Human
Predicted Bovine, Mouse, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46683 Da
Antigen Region 183-210 aa
Additional Information
Gene ID 5080
Other Names Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6, AN2
Target/Specificity This PAX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-210 amino acids from the Central region of human PAX6.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAX6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAX6
Synonyms AN2
Function Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
Cellular Location Nucleus.
Tissue Location Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form
Research Areas
Citations (0)

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PAX6 is paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription.


Firsova,N.V.,, Dokl. Biol. Sci. 426, 264-266 (2009)

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$ 295.00
$ 99.00
Cat# AP6929c
(40 western blots)
Availability: In Stock
Bulk Size
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