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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   C6 Antibody (N-term)   

C6 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - C6 Antibody (N-term) AP6937a
    Western blot analysis of lysate from human blood plasma tissue lysate, using C6 Antibody (N-term)(Cat. #AP6937a). AP6937a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
  • IHC-P - C6 Antibody (N-term) AP6937a
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with C6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - C6 Antibody (N-term) AP6937a
    Flow cytometric analysis of MDA-231 cells using C6 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - C6 Antibody (N-term) AP6937a
    Confocal immunofluorescent analysis of C6 Antibody (N-term)(Cat#AP6937a) with MDA-MB231 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, IF, E
Primary Accession P13671
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 104786 Da
Antigen Region 30-58 aa
Additional Information
Gene ID 729
Other Names Complement component C6, C6
Target/Specificity This C6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human C6.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsC6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name C6
Function Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
Cellular Location Secreted.
Research Areas
Citations ( 0 )

Background

C6 is a component of complement cascade. It is part of the membrane attack complex which can insert into the cell membrane and cause cell to lyse. People with C6 deficiency are prone to bacterial infection.

References

Wu,C., et.al., Proteomics 7 (11), 1775-1785 (2007)

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$ 295.00
$ 99.00
Cat# AP6937a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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