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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   HMHA1 Antibody (Center)   

HMHA1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HMHA1 Antibody (Center) AP6985c
    Western blot analysis of HMHA1 Antibody (Center) (Cat. #AP6985c) in A2058 cell line lysates (35ug/lane). HMHA1 (arrow) was detected using the purified Pab.
  • IHC-P - HMHA1 Antibody (Center) AP6985c
    Formalin-fixed and paraffin-embedded human lymph reacted with HMHA1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - HMHA1 Antibody (Center) AP6985c
    Flow cytometric analysis of CEM cells using HMHA1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q92619
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 124614 Da
Antigen Region 877-903 aa
Additional Information
Gene ID 23526
Other Names Minor histocompatibility protein HA-1, Minor histocompatibility antigen HA-1, mHag HA-1, HMHA1, KIAA0223
Target/Specificity This HMHA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 877-903 amino acids from the Central region of human HMHA1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHMHA1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMHA1
Synonyms KIAA0223
Function GTPase activator for the Rho-type GTPases.
Tissue Location Expressed on cells of the hematopoietic lineage. Detected in dendritic cells and epidermal Langerhans cells. Expressed in peripheral blood mononuclear cells, in all leukemia/lymphoma cell lines. Detected also in some solid tumors and tissues such as cancerous and non-cancerous tissue
Research Areas
Citations (0)

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Background

The minor histocompatibility antigen (mHags), HA-1 is a immunogenic alloantigen shown to be responsible for graft-versus-host disease (GVHD) in HLA-identical bone marrow transplantation. The antigen has two known alleles resulting in a single amino acid polymorphism. The HA-1H allele encodes histidine, whereas the HA-1R allele encodes arginine.

References

Gillespie,G.,et.al., Hematol. J. 1 (6), 403-410 (2000)

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Cat# AP6985c
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(40 western blots)
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