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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GRK1 Antibody (C-term)   

GRK1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GRK1 Antibody (C-term) AP7003a
    The anti-GRK1 Pab (Cat. #AP7003a) is used in Western blot to detect GRK1 in HeLa cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).
  • IHC-P - GRK1 Antibody (C-term) AP7003a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - GRK1 Antibody (C-term) AP7003a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK1 Antibody (C-term) (Cat.#AP7003a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q15835
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 63526 Da
Antigen Region 520-550 aa
Additional Information
Gene ID 6011
Other Names Rhodopsin kinase, RK, G protein-coupled receptor kinase 1, GRK1, RHOK
Target/Specificity This GRK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human GRK1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGRK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GRK1 (HGNC:10013)
Synonyms RHOK
Function Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
Cellular Location Membrane {ECO:0000250|UniProtKB:P28327}; Lipid-anchor {ECO:0000250|UniProtKB:P28327}
Tissue Location Retinal-specific. Expressed in rods and cones cells.
Research Areas
Citations (0)

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Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

References

Zhao, X., et al., J. Biol. Chem. 273(9):5124-5131 (1998).
Zhao, X., et al., Vis. Neurosci. 14(2):225-232 (1997).
Yamamoto, S., et al., Nat. Genet. 15(2):175-178 (1997).
Khani, S.C., et al., Genomics 35(3):571-576 (1996).
Lorenz, W., et al., Proc. Natl. Acad. Sci. U.S.A. 88(19):8715-8719 (1991).

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$ 295.00
$ 99.00
Cat# AP7003a
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(40 western blots)
Availability: In Stock
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