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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ADRBK1 Antibody (C-term)   

ADRBK1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ADRBK1 Antibody (C-term) AP7004a
    Western blot analysis of anti-GRK2 C-term Pab (Cat. #AP7004a) in Ramos cell lysate. GRK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • IHC-P - ADRBK1 Antibody (C-term) AP7004a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - ADRBK1 Antibody (C-term) AP7004a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRK2 Antibody (C-term) (Cat.#AP7004a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25098
Other Accession P26817, Q99MK8, P21146
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 79574 Da
Antigen Region 633-660 aa
Additional Information
Gene ID 156
Other Names Beta-adrenergic receptor kinase 1, Beta-ARK-1, G-protein coupled receptor kinase 2, ADRBK1, BARK, BARK1, GRK2
Target/Specificity This ADRBK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 633-660 amino acids from the C-terminal region of human ADRBK1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADRBK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BARK, BARK1, GRK2
Function Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P21146}. Cell membrane {ECO:0000250|UniProtKB:P21146}
Tissue Location Expressed in peripheral blood leukocytes.
Research Areas
Citations ( 0 )


Beta-adrenergic receptor kinase (ADRBK1), also known as GRK2, phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. ADRBK1 is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Heart failure is accompanied by severely impaired beta-adrenergic receptor (beta-AR) function. An important mechanism for the rapid desensitization of beta-AR function is agonist-stimulated receptor phosphorylation by the beta-AR kinase (beta-ARK1), an enzyme known to be elevated in failing human heart tissue. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.


Li, J., et al., J. Biol. Chem. 278(32):30219-30226 (2003).
Wan, K.F., et al., J. Biol. Chem. 278(20):18658-18663 (2003).
Yang, X.L., et al., World J. Gastroenterol. 9(4):800-803 (2003).
Hagen, S.A., et al., Anesthesiology 98(2):343-348 (2003).
Eichmann, T., et al., J. Biol. Chem. 278(10):8052-8057 (2003).

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$ 295.00
$ 99.00
Cat# AP7004a
(40 western blots)
Availability: In Stock
Bulk Size
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