- CITATIONS: 1
|Application ||WB, E|
|Other Accession||Q7ZXC9, P09330, Q9CS42, Q4R4R7, P11908, P60892, Q9D7G0, Q4R4U3, Q2HJ58|
|Predicted||Bovine, Monkey, Rat, Xenopus|
|Calculated MW||34834 Da|
|Antigen Region||79-109 aa|
|Other Names||Ribose-phosphate pyrophosphokinase 1, PPRibP, Phosphoribosyl pyrophosphate synthase I, PRS-I, PRPS1|
|Target/Specificity||This PRPS1/PRPS2/PRPS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-109 amino acids from the N-terminal region of human PRPS1/PRPS2/PRPS3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PRPS1/PRPS2/PRPS3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.|
Provided below are standard protocols that you may find useful for product applications.
Phosphoribosylpyrophosphate synthetase (PRPS; EC 188.8.131.52) catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for the de novo and salvage pathways of purine, pyrimidine, and pyridine biosynthesis. By PCR of human lymphoblast mRNA using primers based on the cDNA sequence of rat PRS I (Prps1), Roessler et al. (1990) isolated a partial human PRPS1 cDNA. They used this partial cDNA to screen lymphoblast cDNA libraries and isolated additional cDNAs corresponding to the entire PRPS1 coding region. The deduced PRPS1 protein has 318 amino acids.
Roessler, B. J., et al. Nucleic Acids Res. 18: 193 (1990).
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