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KIST (KIS) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - KIST (KIS) Antibody (C-term) AP7063b
    All lanes : Anti-KIST Antibody (L280) at 1:1000 dilution Lane 1: human skeletal muscle lysate Lane 2: A-673 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - KIST (KIS) Antibody (C-term) AP7063b
    The anti-KIST Pab (Cat. #AP7063b) is used in Western blot to detect KIST in K562 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8TAS1
Other Accession Q63285, NP_787062
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46546 Da
Antigen Region 265-295 aa
Additional Information
Gene ID 127933
Other Names Serine/threonine-protein kinase Kist, Kinase interacting with stathmin, PAM COOH-terminal interactor protein 2, P-CIP2, U2AF homology motif kinase 1, UHMK1, KIS, KIST
Target/Specificity This KIST (KIS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-295 amino acids from the C-terminal region of human KIST (KIS).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKIST (KIS) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UHMK1
Synonyms KIS, KIST
Function Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA (By similarity).
Cellular Location Nucleus.
Tissue Location Widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes
Research Areas
Citations (0)

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KIST, a member of the Ser/Thr protein kinase family, is a pyruvate kinase that catalyzes formation of phosphoenolpyruvate from pyruvate and ATP. A role for the primarily nuclear KIST protein in mediation of cellular metabolism has been postulated based on the interaction identified with thyroid hormone. KIST is widely expressed, with highest abundance in skeletal muscle, kidney, placenta and peripheral blood leukocytes. Upon serum stimulation, KIST phosphorylates CDKN1B/p27Kip1, thereby regulating the subcellular location of CDKN1B and cell cycle progression in the G1 phase. KIST, which contains one RNA recognition motif domain, has been proposed to partipate in trafficking and processing of RNA. KIST binds to Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells.


Bieche, I., et al., Brain Res. Mol. Brain Res. 114(1):55-64 (2003).
Boehm, M., et al., EMBO J. 21(13):3390-3401 (2002).
Caldwell, B.D., et al., J. Biol. Chem. 274(49):34646-34656 (1999).

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$ 295.00
$ 99.00
Cat# AP7063b
(40 western blots)
Availability: In Stock
Bulk Size
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