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PGK1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

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  • IHC-P - PGK1 Antibody (Center) AP7094b
    Immunohistochemical analysis of paraffin-embedded H. kidney section using PGK1 Antibody (Center)(Cat#AP7094b). AP7094b was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • WB - PGK1 Antibody (Center) AP7094b
    Western blot analysis of lysates from Hela, U-937 cell line (from left to right), using PGK1 Antibody (G132)(Cat. #AP7094b). AP7094b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • IHC-P - PGK1 Antibody (Center) AP7094b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - PGK1 Antibody (Center) AP7094b
    Confocal immunofluorescent analysis of PGK1 Antibody (Center)(Cat#AP7094b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • FC - PGK1 Antibody (Center) AP7094b
    PGK1 Antibody (Center) (Cat. #AP7094b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00558
Other Accession P16617, P09411, Q60HD8, P00559
Reactivity Human, Mouse
Predicted Rat, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44615 Da
Antigen Region 117-145 aa
Additional Information
Gene ID 5230
Other Names Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA
Target/Specificity This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the Central region of human PGK1.
Dilution IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPGK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PGK1
Synonyms PGKA
Function In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
Cellular Location Cytoplasm.
Research Areas
Citations ( 0 )


Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.


Lay, A. J., et al. Nature 408: 869-873 (2000).

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$ 295.00
$ 99.00
Cat# AP7094b
(40 western blots)
Availability: In Stock
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