|Application ||WB, E|
|Calculated MW||74806 Da|
|Antigen Region||573-604 aa|
|Other Names||Serine/threonine-protein kinase Nek8, Never in mitosis A-related kinase 8, NimA-related protein kinase 8, Nima-related protein kinase 12a, NEK8, JCK, NEK12A|
|Target/Specificity||This NEK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 573-604 amino acids from the C-terminal region of human NEK8.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NEK8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia (By similarity). Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, cilium Note=Predominantly cytoplasmic. Localizes to the proximal region of the primary cilium and is not observed in dividing cells|
|Tissue Location||Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma|
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Provided below are standard protocols that you may find useful for product applications.
NEK8 is a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans.
Valkova,N. et al. Mol. Cell Proteomics 4 (7), 1009-1018 (2005)
Bowers,A.J. et al. Gene 328, 135-142 (2004)
Holland,P.M. et al. J. Biol. Chem. 277 (18), 16229-16240 (2002)
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