- CITATIONS: 2
|Application ||IHC-P, WB, E|
|Calculated MW||38500 Da|
|Antigen Region||314-343 aa|
|Other Names||Calcium/calmodulin-dependent protein kinase type 1B, CaM kinase I beta, CaM kinase IB, CaM-KI beta, CaMKI-beta, Pregnancy up-regulated non-ubiquitously-expressed CaM kinase, PNCK|
|Target/Specificity||This PNCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the C-terminal region of human PNCK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PNCK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 (By similarity).|
|Cellular Location||Cytoplasm. Nucleus|
Provided below are standard protocols that you may find useful for product applications.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Gardner, H.P., et al., Cancer Res. 60(19):5571-5577 (2000).
Gardner, H.P., et al., Genomics. 63(2):279-288 (2000).
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