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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Pyruvate Kinase (PKM2) Antibody (C-term N491)   

Pyruvate Kinase (PKM2) Antibody (C-term N491)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Pyruvate Kinase (PKM2) Antibody (C-term N491) AP7173A
    All lanes : Anti-PKM2 Antibody (N491) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Pyruvate Kinase (PKM2) Antibody (C-term N491) AP7173A
    All lanes : Anti-PKM2 Antibody (N491) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Pyruvate Kinase (PKM2) Antibody (C-term N491) AP7173A
    Western blot analysis of hPKM2-N491 (Cat.# AP7173a) in A2058 cell line(lane 1) and mouse brain tissue(lane 2) lysates (35ug/lane). PKM2 (arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P14618
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57937 Da
Antigen Region 476-505 aa
Additional Information
Gene ID 5315
Other Names Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP3, PK2, PK3, PKM2
Target/Specificity This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPyruvate Kinase (PKM2) Antibody (C-term N491) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PKM
Synonyms OIP3, PK2, PK3, PKM2
Function Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Cellular Location Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity
Tissue Location Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
Research Areas
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Background

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

References

Lehner, B., et al., Genome Res. 14(7):1315-1323 (2004).
Gevaert, K., et al., Nat. Biotechnol. 21(5):566-569 (2003).
Valentini, G., et al., J. Biol. Chem. 277(26):23807-23814 (2002).
Lowrie, D.J. Jr., et al., J. Struct. Biol. 132(2):83-94 (2000).
Williams, J.M., et al., Mol. Microbiol. 27(1):171-186 (1998).

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$ 295.00
$ 99.00
Cat# AP7173A
Size:
Quantity:
(40 western blots)
Availability: In Stock
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