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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PSKH1 Antibody (N-term H70)   

PSKH1 Antibody (N-term H70)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PSKH1 Antibody (N-term H70) AP7179c
    Western blot analysis of anti-PSKH1 Antibody (N-term H70) (Cat.#AP7179c) in Hela cell line lysate and mouse kidney tissue lysate (35ug/lane). PSKH1 (arrow) was detected using the purified Pab.
  • IHC-P - PSKH1 Antibody (N-term H70) AP7179c
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P11801
Other Accession Q0V7M1
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48035 Da
Antigen Region 55-91 aa
Additional Information
Gene ID 5681
Other Names Serine/threonine-protein kinase H1, Protein serine kinase H1, PSK-H1, PSKH1
Target/Specificity This PSKH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-91 amino acids from the N-terminal region of human PSKH1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSKH1 Antibody (N-term H70) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSKH1
Function May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.
Cellular Location Golgi apparatus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus speckle Endoplasmic reticulum membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Cytoplasm. Note=Localized in the brefeldin A- sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells The acylation-negative form may be only cytoplasmic and nuclear Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes. Dual acylation is required to stabilize the interaction with Golgi membranes
Tissue Location Expressed in all tissues and cell lines tested with the highest level of abundance in testis
Research Areas
Citations (0)

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Background

PSKH1 may be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing. PSKH1 is localized in the Brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm.

References

Brede, G., et al., Exp. Cell Res. 291(2):299-312 (2003).
Brede, G., et al., Nucleic Acids Res. 30(23):5301-5309 (2002).
Brede, G., et al., Genomics 70(1):82-92 (2000).
Amarzguioui, M., et al., Nucleic Acids Res. 28(21):4113-4124 (2000).
Larsen, F., et al., Hum. Mol. Genet. 2(10):1589-1595 (1993).

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$ 295.00
$ 99.00
Cat# AP7179c
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Quantity:
(40 western blots)
Availability: In Stock
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