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AMPK alpha 1 Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP7201a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
AMPK alpha 1 Antibody (C-term) - Product info | |
| Application | WB
|
| Primary Accession | Q13131 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 64009 Da |
AMPK alpha 1 Antibody (C-term) - Additional info | |
| Gene ID 5562 | |
| Other Names PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase; Tau-protein kinase PRKAA1 | |
| Target/Specificity This AMPK alpha 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487~517 amino acids from the C-terminal region of human AMPK alpha 1. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AMPK alpha 1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
AMPK alpha 1 Antibody (C-term) - Protein Information | |
| Name PRKAA1 | |
| Synonyms AMPK1 | |
| Function Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1 | |
| Cellular Location Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters | |
AMPK alpha 1 Antibody (C-term) - Related products
AP7260a: AMPK alpha (PRKAA1) Antibody (S487)
AP7260b: AMPK alpha (PRKAA1) Antibody (N-term)
AM1858a: PRKAA1 Antibody (Ascites)
AP3700a: Phospho-PRKAA1 -S487 Antibody
AP7201a: AMPK alpha 1 Antibody (C-term)
AP7255a: AMPK1 Antibody (N-term)
RI14207: PRKAA1 predesign siRNA
DC07754: Human PRKAA1 cDNA Clone
BP3700a: Phospho-PRKAA1-S487 Antibody Blocking Peptide
BP7201a: AMPK alpha 1 Antibody (C-term) Blocking Peptide
BP7255a: AMPK1 Antibody (N-term) Blocking Peptide
BP7260a: PRKAA1 Antibody (S487) Blocking Peptide
AT3426a: PRKAA1 Antibody (monoclonal) (M03)
AJ1037a: AMPK alpha 1 Antibody
AMPK alpha 1 Antibody (C-term) - Application data
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Western blot analysis of anti-AMPK alpha 1 C-term Pab (Cat. #AP7201a) in mouse liver lysate. AMPK alpha 1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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AMPKalpha1 Antibody (C494) (Cat. #AP7201a) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the AMPKalpha1 antibody detected the AMPKalpha1 protein (arrow).
AMPK alpha 1 Antibody (C-term) - Research Areas
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BACKGROUND
AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.
REFERENCES
Zhang, Q.H., et al., Genome Res. 10(10):1546-1560 (2000). Stapleton, D., et al., J. Biol. Chem. 271(2):611-614 (1996).
