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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Cdc25B Antibody (N-term) (A166)   

Cdc25B Antibody (N-term) (A166)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Cdc25B Antibody (N-term) (A166) AP7256d
    Western blot analysis of Cdc25B Antibody (N-term)(A166)(Cat# AP7256d) in A2058 cell line lysates (35ug/lane). Cdc25B (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30305
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 64987 Da
Antigen Region 165-194 aa
Additional Information
Gene ID 994
Other Names M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2
Target/Specificity This Cdc25B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the N-terminal region of human Cdc25B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCdc25B Antibody (N-term) (A166) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC25B
Synonyms CDC25HU2
Function Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole
Research Areas
Citations (0)

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CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.


Uchida, S., et al., Biochem. Biophys. Res. Commun. 316(1):226-232 (2004).
Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004).
Wu, W., et al., Cancer Res. 63(19):6195-6199 (2003).
Mils, V., et al., Exp. Cell Res. 285(1):99-106 (2003).
Theis-Febvre, N., et al., Oncogene 22(2):220-232 (2003).

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$ 295.00
$ 99.00
Cat# AP7256d
(40 western blots)
Availability: In Stock
Bulk Size
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