|Application ||WB, IHC-P, E|
|Other Accession||Q8K3X0, A5D7H5, NP_619601, Q8K3W3|
|Predicted||Bovine, Mouse, Rat|
|Calculated MW||76278 Da|
|Antigen Region||206-236 aa|
|Other Names||Protein CASC3, Cancer susceptibility candidate gene 3 protein homolog, Metastatic lymph node gene 51 protein homolog, MLN 51 homolog, Protein barentsz, Btz, mBtz, Casc3, Mln51|
|Target/Specificity||This CASC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids of human CASC3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CASC3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA- helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer.|
|Cellular Location||Cytoplasm, perinuclear region. Nucleus. Nucleus speckle. Note=Predominantly found in the perinuclear region (By similarity). Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Shuttles between the cytoplasm and the stress granules. More specifically found in nuclear intrachromatin granules clusters (IGC), also called nuclear speckles, which are storage compartments for nuclear proteins involved in mRNA processing. Colocalizes in nuclear speckles with MAGOH. Under stress condition, colocalizes with FMR1 and TIA1, but not MAGOH and RBM8A EJC core factors, in cytoplasmic stress granules, which contain stored mRNAs whose translation is stopped in response to stress.|
|Tissue Location||Widely expressed. Overexpressed in breast cancers and metastasis, as well as in gastric cancers|
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Provided below are standard protocols that you may find useful for product applications.
Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport.
Macchi,P., J. Neurosci. 23 (13), 5778-5788 (2003)
Degot,S., Oncogene 21 (28), 4422-4434 (2002)
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