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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PHB2 Antibody (Y248)   

PHB2 Antibody (Y248)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PHB2 Antibody (Y248) AP7270e
    Western blot analysis of PHB2-Y248(Cat. #AP7270e) in MDA-MB435 cell line lysates (35ug/lane).PHB2 (arrow) was detected using the purified Pab.
  • WB - PHB2 Antibody (Y248) AP7270e
    Western blot analysis of PHB2-Y248 (Cat. #AP7270e) in mouse kidney tissue lysates (35ug/lane). PHB2 (arrow) was detected using the purified Pab.
  • WB - PHB2 Antibody (Y248) AP7270e
    Western blot analysis of PHB2 (arrow) using PHB2 Antibody (Y248) (Cat. #AP7270e). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2) (Origene Technologies).
  • IHC-P - PHB2 Antibody (Y248) AP7270e
    PHB2 Antibody (Y248)(Cat. #AP7270e) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHB2 Antibody (Y248) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q99623
Other Accession Q5XIH7, O35129, Q2HJ97, NP_009204
Reactivity Human, Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33296 Da
Antigen Region 225-255 aa
Additional Information
Gene ID 11331
Other Names Prohibitin-2, B-cell receptor-associated protein BAP37, D-prohibitin, Repressor of estrogen receptor activity, PHB2 {ECO:0000312|EMBL:AAH147661, ECO:0000312|HGNC:HGNC:30306}
Target/Specificity This PHB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-255 amino acids from human PHB2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHB2 Antibody (Y248) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PHB2 {ECO:0000312|EMBL:AAH14766.1, ECO:0000312|HGNC:HGNC:30306}
Function Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.
Cellular Location Mitochondrion inner membrane. Cytoplasm. Nucleus. Note=Also cytoplasmic and nuclear.
Research Areas
Citations (0)

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Background

PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. It functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity and is probably involved in regulating mitochondrial respiration activity and in aging.

References

Takata,H., Curr. Biol. 17 (15), 1356-1361 (2007)
Kasashima,K., J. Biol. Chem. 281 (47), 36401-36410 (2006)

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$ 295.00
$ 99.00
Cat# AP7270e
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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