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NPTX1-Y344 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - NPTX1-Y344 Antibody AP7272d
    AP7272d staining NPTX1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - NPTX1-Y344 Antibody AP7272d
    AP7272d staining NPTX1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - NPTX1-Y344 Antibody AP7272d
    All lanes : Anti-NPTX1-Y344 Antibody at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - NPTX1-Y344 Antibody AP7272d
    All lanes : Anti-NPTX1-Y344 Antibody at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - NPTX1-Y344 Antibody AP7272d
    All lanes : Anti-NPTX1-Y344 Antibody at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - NPTX1-Y344 Antibody AP7272d
    All lanes : Anti-NPTX1Y344 Antibody at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - NPTX1-Y344 Antibody AP7272d
    Western blot analysis of NPTX1-Y344 (Cat. #AP7272d) in mouse brain tissue lysates (35ug/lane).NPTX1 (arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q15818
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 47122 Da
Antigen Region 322-350 aa
Additional Information
Gene ID 4884
Other Names Neuronal pentraxin-1, NP1, Neuronal pentraxin I, NP-I, NPTX1
Target/Specificity This NPTX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from human NPTX1.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNPTX1-Y344 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NPTX1
Function May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.
Cellular Location Cytoplasmic vesicle, secretory vesicle
Research Areas
Citations (0)

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Background

NPTX1 is a member of the neuronal pentraxin family. Human neuronal pentraxin 1 is similar to rat NP1 which is a binding protein for the snake venom toxin taipoxin.

References

Hossain,M.A., J. Neurosci. 24 (17), 4187-4196 (2004)
Schlimgen,A.K., Neuron 14 (3), 519-526 (1995)

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$ 295.00
$ 99.00
Cat# AP7272d
Size:
Quantity:
(40 western blots)
Availability: In Stock
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