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EN1 (Engrailed 1) Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EN1 (Engrailed 1) Antibody (N-term) AP7278a
    All lanes : Anti-EN1 Antibody (N-term) at 1:1000 dilution Lane 1: human fetal brain lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - EN1 (Engrailed 1) Antibody (N-term) AP7278a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (Human Sarcoma cell line) cells labeling Pdx1 with AP7278a at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Immunofluorescence image showing nucleus and nucleoli staining on U-2 OS cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).
  • IHC-P - EN1 (Engrailed 1) Antibody (N-term) AP7278a
    AP7278a staining EN1 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - EN1 (Engrailed 1) Antibody (N-term) AP7278a
    All lanes : Anti-EN1 Antibody (N-term) at 1:2000 dilution Lane 1: NCI-H460 whole cell lysates Lane 2: SH-SY5Y whole cell lysates Lane 3: PC-3 whole cell lysates Lane 4: Jurkat whole cell lysates Lane 5: 293T whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - EN1 (Engrailed 1) Antibody (N-term) AP7278a
    Western blot analysis of lysates from 293, SH-SY5Y, NCI-H460 cell line (from left to right), using EN1 Antibody (N-term)(Cat. #AP7278a). AP7278a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • IF - EN1 (Engrailed 1) Antibody (N-term) AP7278a
    Fluorescent image of U251 cell stained with EN1 Antibody (N-term)(Cat#AP7278a).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).EN1 immunoreactivity is localized to Nucleus significantly.
  • IHC-P - EN1 (Engrailed 1) Antibody (N-term) AP7278a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term) (Cat.#AP7278a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q05925
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40115 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 2019
Other Names Homeobox protein engrailed-1, Homeobox protein en-1, Hu-En-1, EN1
Target/Specificity This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEN1 (Engrailed 1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EN1
Cellular Location Nucleus.
Research Areas
Citations (0)

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Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.


Bachar-Dahan,L., Mol. Biol. Cell 17 (6), 2572-2580 (2006)
Kohler,A., Genomics 15 (1), 233-235 (1993)

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$ 295.00
$ 99.00
Cat# AP7278a
(40 western blots)
Availability: In Stock
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