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SERPINF1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SERPINF1 Antibody (N-term) AP7315A
    Anti-SERPINF1 Antibody (N-term)at 1:1000 dilution + human blood plasma lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SERPINF1 Antibody (N-term) AP7315A
    Western blot analysis of SERPINF1 Antibody (N-term) (Cat.# AP7315a) in 293 cell line lysates (35ug/lane). SERPINF1 (arrow) was detected using the purified Pab.
  • IHC-P - SERPINF1 Antibody (N-term) AP7315A
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SERPINF1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - SERPINF1 Antibody (N-term) AP7315A
    SERPINF1 Antibody (N-term)(Cat. #AP7315a) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P36955
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46312 Da
Antigen Region 9-36 aa
Additional Information
Gene ID 5176
Other Names Pigment epithelium-derived factor, PEDF, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1, SERPINF1, PEDF
Target/Specificity This SERPINF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-36 amino acids from the N-terminal region of human SERPINF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSERPINF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PEDF
Function Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Cellular Location Secreted. Melanosome. Note=Enriched in stage I melanosomes
Tissue Location Retinal pigment epithelial cells and blood plasma.
Research Areas
Citations (0)

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SERPINF1 is a member of the serpin family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.


Bessho,H., Kondo,N. Mol. Vis. 15, 1107-1114 (2009)
Petersen,S.V., Valnickova,Z. Biochem. J. 374 (PT 1), 199-206 (2003)
Tombran-Tink,J., Pawar,H. Genomics 19 (2), 266-272 (1994)

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Cat# AP7315A
(40 western blots)
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