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PITX1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PITX1 Antibody (Center) AP7334c
    Western blot analysis of PITX1 antibody (Center) (Cat.#AP7334c) in NCI-H460 cell line lysates (35ug/lane). PITX1 (arrow) was detected using the purified Pab.
  • IHC-P - PITX1 Antibody (Center) AP7334c
    PITX1 Antibody (Center) (RB18913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PITX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - PITX1 Antibody (Center) AP7334c
    PITX1 Antibody (Center) (Cat. #AP7334c) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - PITX1 Antibody (Center) AP7334c
    Western blot analysis of PITX1 (arrow) using rabbit polyclonal PITX1 Antibody (Center) (Cat. #AP7334c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P78337
Other Accession P52906, O18400, Q9I8K3, Q6QU75, Q9PWR3, Q9R0W1, P97474, Q99697, Q9W5Z2, O93385, Q9W751, Q99NA7, P70314, P56673
Reactivity Human
Predicted Chicken, Mouse, Rat, Xenopus, Zebrafish, Drosophila, C.Elegans
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34128 Da
Antigen Region 123-150 aa
Additional Information
Gene ID 5307
Other Names Pituitary homeobox 1, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, Paired-like homeodomain transcription factor 1, PITX1, BFT, PTX1
Target/Specificity This PITX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-150 amino acids from the Central region of human PITX1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPITX1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PITX1
Synonyms BFT, PTX1
Function May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
Cellular Location Nucleus.
Research Areas
Citations (0)

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PITX1 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin.


Grimminger,P., Ling,F.C. Anticancer Res. 29 (4), 1281-1286 (2009)
Gurnett,C.A., Alaee,F. Am. J. Hum. Genet. 83 (5), 616-622 (2008)
Philippi,A., Tores,F. BMC Med. Genet. 8, 74 (2007)
Shang,J., Li,X. Genomics 40 (1), 108-113 (1997)

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Cat# AP7334c
(40 western blots)
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