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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   VRK1 Antibody (Center)   

VRK1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - VRK1 Antibody (Center) AP7408c
    All lanes : Anti-VRK1(E381) antibody at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: 293T/17 whole cell lysates Lane 3: MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99986
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 45476 Da
Antigen Region 366-396 aa
Additional Information
Gene ID 7443
Other Names Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1, VRK1
Target/Specificity This VRK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-396 amino acids from the Central region of human VRK1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVRK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VRK1
Function Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr- 18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis
Tissue Location Widely expressed. Highly expressed in fetal liver, testis and thymus.
Research Areas
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This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.


Nezu, J., et al., Genomics 45(2):327-331 (1997).

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$ 295.00
$ 99.00
Cat# AP7408c
(40 western blots)
Availability: In Stock
Bulk Size
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