|Application ||WB, E|
|Calculated MW||32541 Da|
|Antigen Region||270-301 aa|
|Other Names||Protein sprouty homolog 4, Spry-4, SPRY4|
|Target/Specificity||This SPRY4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-301 amino acids from the C-terminal region of human SPRY4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPRY4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1.|
|Cellular Location||Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side|
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Provided below are standard protocols that you may find useful for product applications.
SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. The protein is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.
Tsai CF, et al. (2008) J Proteome Res
Guo A, et al. (2008) Proc Natl Acad Sci U S A 105, 692-7
Rikova K, et al. (2007) Cell 131, 1190-203
Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM (2007) Proc Natl Acad Sci U S A 104, 5860-5
Tsumura Y, et al. (2005) Biochem J 387, 627-37
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