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DDX5 Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP7459c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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DDX5 Antibody (Center) - Product info | |
| Application | WB, IHC
|
| Primary Accession | P17844 |
| Other Accession | Q61656, Q4R6M5 |
| Reactivity | Human |
| Predicted | Mouse, Monkey |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 69148 Da |
DDX5 Antibody (Center) - Additional info | |
| Gene ID 1655 | |
| Other Names DDX5; G17P1; HELR; HLR1; Probable ATP-dependent RNA helicase DDX5; DEAD box protein 5; RNA helicase p68 | |
| Target/Specificity This DDX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313~342 amino acids from the Center region of human DDX5. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions DDX5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
DDX5 Antibody (Center) - Protein Information | |
| Name DDX5 | |
| Synonyms G17P1, HELR, HLR1 | |
| Function Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10 The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1 | |
| Cellular Location Nucleus, nucleolus. | |
DDX5 Antibody (Center) - Related products
AP7459b: DDX5 Antibody (C-term)
AP7459c: DDX5 Antibody (Center)
DC04253: Human DDX5 cDNA Clone
LY11973a: DDX5 Over-expression Lysate
BP7459b: DDX5 Antibody (C-term) Blocking Peptide
DDX5 Antibody (Center) - Application data
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Western blot analysis of DDX5 antibody (Center) (Cat.# AP7459c) in Romas cell line lysates (35ug/lane). DDX5 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human brain with DDX5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
DDX5 Antibody (Center) - Research Areas
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BACKGROUND
DDX5 is putative RNA helicases. The protein is implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This protein is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen.
REFERENCES
Ong S.E., Mittler G.Nat. Methods 1:119-126(2004) Daub H., Olsen J.V.Mol. Cell 31:438-448(2008)
