|Application ||WB, IHC-P, E|
|Calculated MW||49307 Da|
|Antigen Region||103-131 aa|
|Other Names||Ig mu chain C region, IGHM|
|Target/Specificity||This IGHM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-131 amino acids from the N-terminal region of human IGHM.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||IGHM Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane- bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as a homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts.|
|Cellular Location||Isoform 1: Secreted. Note=During differentiation, B-lymphocytes switch from expression of membrane- bound IgM to secretion of IgM|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Dorai H., Gillies S.D.Nucleic Acids Res. 17:6412-6412(1989)
Rabbitts T.H.Nucleic Acids Res. 9:4509-4524(1981)
Watanabe S.Hoppe-Seyler's Z. Physiol. Chem. 354:1505-1509(1973)
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