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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CAPZA1 Antibody (N-term)   

CAPZA1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CAPZA1 Antibody (N-term) AP7488A
    All lanes : Anti-CAPZA1 Antibody (N-term) at 1:4000 dilution Lane 1: A431 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CAPZA1 Antibody (N-term) AP7488A
    Western blot analysis of CAPZA1 antibody (N-term) (Cat.# AP7488a) in HL60 cell line lysates (35ug/lane). CAPZA1 (arrow) was detected using the purified Pab.
  • IF - CAPZA1 Antibody (N-term) AP7488A
    Immunofluorescence analysis of CAPZA1 Antibody (N-term) with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
  • FC - CAPZA1 Antibody (N-term) AP7488A
    CAPZA1 Antibody (N-term) (Cat. #AP7488a) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P52907
Other Accession Q4R959
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 32923 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 829
Other Names F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1
Target/Specificity This CAPZA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CAPZA1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCAPZA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)

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CAPZA1 is a member of the F-actin capping protein alpha subunit family. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments.


Maun,N.A., Speicher,D.W. Biochemistry 35 (11), 3518-3524 (1996)
Barron-Casella,E.A., Torres,M.A. J. Biol. Chem. 270 (37), 21472-21479 (1995)
Canton,D.A., Olsten,M.E. J. Biol. Chem. 281 (47), 36347-36359 (2006)

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$ 295.00
$ 99.00
Cat# AP7488A
(40 western blots)
Availability: In Stock
Bulk Size
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