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>   home   >   Products   >   Primary Antibodies   >   AlaRS (AARS) Antibody (N-term)   

AlaRS (AARS) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - AlaRS (AARS) Antibody (N-term) AP7558a
    Western blot analysis of anti-AARS Pab (Cat.#AP7558a) in K562 cell line lysates (35ug/lane).AARS (arrow) was detected using the purified Pab.
  • IHC-P - AlaRS (AARS) Antibody (N-term) AP7558a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AARS antibody (N-term) (Cat.#AP7558a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49588
Other Accession Q23122, P50475, Q8BGQ7, Q9VLM8, O01541
Reactivity Human
Predicted C.Elegans, Drosophila, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 106810 Da
Antigen Region 61-91 aa
Additional Information
Gene ID 16
Other Names Alanine--tRNA ligase, cytoplasmic {ECO:0000255|HAMAP-Rule:MF_03133}, 6117 {ECO:0000255|HAMAP-Rule:MF_03133}, Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133}, AlaRS {ECO:0000255|HAMAP-Rule:MF_03133}, Renal carcinoma antigen NY-REN-42, AARS {ECO:0000255|HAMAP-Rule:MF_03133}
Target/Specificity This AlaRS (AARS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the N-terminal region of human AlaRS (AARS).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAlaRS (AARS) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AARS {ECO:0000255|HAMAP-Rule:MF_03133}
Function Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03133}.
Citations (0)

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The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases,of the class II enzymes.Class II tRNA synthases evolved early in evolution and are highly conserved.This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein.tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons.They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.


Shiba,K.,Biochemistry 34 (33), 10340-10349 (1995)
Ripmaster,T.L.,Proc. Natl. Acad. Sci. U.S.A. 92 (11), 4932-4936 (1995)
Sang Lee,J.,Cell 127 (3), 635-648 (2006)

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$ 295.00
$ 99.00
Cat# AP7558a
(40 western blots)
Availability: In Stock
Bulk Size
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