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AlaRS (AARS) Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P49588 |
| Other Accession | Q23122, P50475, Q8BGQ7, Q9VLM8, O01541 |
| Reactivity | Human |
| Predicted | C.Elegans, Drosophila, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 106810 Da |
| Antigen Region | 61-91 aa |
| Gene ID | 16 |
|---|---|
| Other Names | Alanine--tRNA ligase, cytoplasmic {ECO:0000255|HAMAP-Rule:MF_03133}, 6117 {ECO:0000255|HAMAP-Rule:MF_03133}, Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133}, AlaRS {ECO:0000255|HAMAP-Rule:MF_03133}, Renal carcinoma antigen NY-REN-42, AARS {ECO:0000255|HAMAP-Rule:MF_03133} |
| Target/Specificity | This AlaRS (AARS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the N-terminal region of human AlaRS (AARS). |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | AlaRS (AARS) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AARS1 |
|---|---|
| Synonyms | AARS {ECO:0000255|HAMAP-Rule:MF_03133} |
| Function | Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala) (PubMed:27622773, PubMed:27911835, PubMed:28493438, PubMed:33909043). Also edits incorrectly charged tRNA(Ala) via its editing domain (PubMed:27622773, PubMed:27911835, PubMed:28493438). |
| Cellular Location | Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03133, ECO:0000269|PubMed:27911835} |
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Provided below are standard protocols that you may find useful for product applications.
Background
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases,of the class II enzymes.Class II tRNA synthases evolved early in evolution and are highly conserved.This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein.tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons.They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
References
Shiba,K.,Biochemistry 34 (33), 10340-10349 (1995)
Ripmaster,T.L.,Proc. Natl. Acad. Sci. U.S.A. 92 (11), 4932-4936 (1995)
Sang Lee,J.,Cell 127 (3), 635-648 (2006)
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