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AARS2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - AARS2 Antibody (Center) AP7559c
    AARS2 Antibody (Center) (Cat.#AP7559c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the AARS2 antibody detected the AARS2 protein (arrow).
  • IHC-P - AARS2 Antibody (Center) AP7559c
    AARS2 Antibody (Center) (Cat. #AP7559c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AARS2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - AARS2 Antibody (Center) AP7559c
    AARS2 Antibody (Center) (Cat. #AP7559c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5JTZ9
Other Accession A2RRN5
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 107340 Da
Antigen Region 366-394 aa
Additional Information
Gene ID 57505
Other Names Alanine--tRNA ligase, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03133}, 6117 {ECO:0000255|HAMAP-Rule:MF_03133}, Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133}, AlaRS {ECO:0000255|HAMAP-Rule:MF_03133}, AARS2 {ECO:0000255|HAMAP-Rule:MF_03133}
Target/Specificity This AARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-394 amino acids from the Central region of human AARS2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAARS2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AARS2 {ECO:0000255|HAMAP-Rule:MF_03133}
Function Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Cellular Location Mitochondrion {ECO:0000255|HAMAP- Rule:MF_03133, ECO:0000269|PubMed:21549344}
Citations (0)

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Alanyl-tRNA synthetase 2 (AARS2) is an enzyme that catalyzes the chemical reaction: ATP + L-alanine + tRNAAla <---> AMP + diphosphate + L-alanyl-tRNAAla. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis.


Bonnefond,L.,Biochemistry 44 (12), 4805-4816 (2005)
Nakayama,M., Genome Res. 12 (11), 1773-1784 (2002)

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Cat# AP7559c
(40 western blots)
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