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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ACAT1 Antibody (C-term)   

ACAT1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - ACAT1 Antibody (C-term) AP7560b
    All lanes : Anti-ACAT1 Antibody (C-term) at 1:2000 dilution Lane 1: SW620 whole cell lysates Lane 2: mouse liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45. 2 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ACAT1 Antibody (C-term) AP7560b
    All lanes : Anti-ACAT1 Antibody (C-term) at 1:1000 dilution Lane 1: SK-BR-3 whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45. 2 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ACAT1 Antibody (C-term) AP7560b
    Western blot analysis of anti-ACAT1 Pab (Cat.#AP7560b) in SK-BR-3 cell line lysates (35ug/lane).ACAT1 (arrow) was detected using the purified Pab.
  • IHC-P - ACAT1 Antibody (C-term) AP7560b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) (Cat.#AP7560b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P24752
Other Accession Q8HXY6
Reactivity Human, Mouse
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 45200 Da
Antigen Region 296-329 aa
Additional Information
Gene ID 38
Other Names Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT
Target/Specificity This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-329 amino acids from the C-terminal region of human ACAT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACAT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACAT1
Synonyms ACAT, MAT
Function Plays a major role in ketone body metabolism.
Cellular Location Mitochondrion.
Research Areas
Citations (0)

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ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.


Locke,J.A.,Prostate 68 (1), 20-33 (2008)
Guo,Z.Y.,Biochemistry 46 (35), 10063-10071 (2007)
Haapalainen,A.M.,Biochemistry 46 (14), 4305-4321 (2007)

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$ 295.00
$ 99.00
Cat# AP7560b
(40 western blots)
Availability: In Stock
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