|Application ||WB, IHC-P, E|
|Calculated MW||170591 Da|
|Antigen Region||285-314 aa|
|Other Names||Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase, Cell proliferation-inducing gene 32 protein, Glutamatyl-prolyl-tRNA synthetase, Glutamate--tRNA ligase, Glutamyl-tRNA synthetase, GluRS, Proline--tRNA ligase, Prolyl-tRNA synthetase, EPRS, GLNS, PARS, QARS, QPRS|
|Target/Specificity||This EPRS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-314 amino acids from the N-terminal region of human EPRS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EPRS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||GLNS, PARS, QARS, QPRS|
|Function||Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop- containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. EPRS is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species.
Jia,J., Mol. Cell 29 (6), 679-690 (2008)
Beausoleil,S.A., Nat. Biotechnol. 24 (10), 1285-1292 (2006)
Kato,T., Cancer Res. 65 (13), 5638-5646 (2005)
Sampath,P., Cell 119 (2), 195-208 (2004)
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