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EPRS Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EPRS Antibody (N-term) AP7565a
    All lanes : Anti-EPRS Antibody (N-term) at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 171 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - EPRS Antibody (N-term) AP7565a
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EPRS antibody (N-term) (Cat.#AP7565a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07814
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 170591 Da
Antigen Region 285-314 aa
Additional Information
Gene ID 2058
Other Names Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase, Cell proliferation-inducing gene 32 protein, Glutamatyl-prolyl-tRNA synthetase, Glutamate--tRNA ligase, Glutamyl-tRNA synthetase, GluRS, Proline--tRNA ligase, Prolyl-tRNA synthetase, EPRS, GLNS, PARS, QARS, QPRS
Target/Specificity This EPRS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-314 amino acids from the N-terminal region of human EPRS.
Dilution WB~~1:8000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEPRS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop- containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Cellular Location Cytoplasm.
Citations (0)

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Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. EPRS is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species.


Jia,J., Mol. Cell 29 (6), 679-690 (2008)
Beausoleil,S.A., Nat. Biotechnol. 24 (10), 1285-1292 (2006)
Kato,T., Cancer Res. 65 (13), 5638-5646 (2005)
Sampath,P., Cell 119 (2), 195-208 (2004)

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$ 295.00
$ 99.00
Cat# AP7565a
(40 western blots)
Availability: In Stock
Bulk Size
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