|Application ||WB, E|
|Other Accession||Q27965, Q27975|
|Calculated MW||70052 Da|
|Antigen Region||503-532 aa|
|Other Names||Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70|
|Target/Specificity||This HSPA1A/HSPA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 503-532 amino acids from human HSPA1A/HSPA1B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HSPA1A/HSPA1B Antibody (Y525) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation (PubMed:23973223).|
|Cellular Location||Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs|
|Tissue Location||HSPA1B is testis-specific.|
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Provided below are standard protocols that you may find useful for product applications.
HSPA1A is a member of the heat shock protein 70 family. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.
Mueller, T., et al., Transplantation 78(2):292-295 (2004).
Fekete, A., et al., Pediatr. Res. 54(4):452-455 (2003).
Broquet, A.H., et al., J. Biol. Chem. 278(24):21601-21606 (2003).
Bruce, C.R., et al., Diabetes 52(9):2338-2345 (2003).
Anwar, A., et al., J. Biol. Chem. 277(16):14060-14067 (2002).
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