|Application ||WB, E|
|Other Accession||P60764, P60763, Q05144, P15153, P48554, Q9TU25, Q6RUV5, P63001, P40792, Q03206, P62998, Q8CFN2, Q007T2, P60766, Q4R4R6, P60953, P40793, Q90694, Q05062, Q2KJ93|
|Predicted||Bovine, C.Elegans, Chicken, Drosophila, Monkey, Mouse, Pig|
|Calculated MW||21450 Da|
|Antigen Region||49-78 aa|
|Other Names||Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, RAC1, TC25|
|Target/Specificity||This RAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from human RAC1.|
|Precautions||RAC1 Antibody (S71) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.|
|Cellular Location||Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Cytoplasm. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts (By similarity).|
|Tissue Location||Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues|
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Provided below are standard protocols that you may find useful for product applications.
RAC1 is a GTPase belonging to the RAS superfamily of small GTP-binding proteins. It is a pleiotropic regulator of many cellular processes, including the cell cycle, cell-cell adhesion, motility (through the actin network), and of epithelial differentiation (proposed to be necessary for maintaining epidermal stem cells).
Wang,T.,Neurosci. Lett. 437 (2), 71-75 (2008)
Gorshkova,I., J. Biol. Chem. 283 (17), 11794-11806 (2008)
Simeone-Penney,M.C.,Am. J. Physiol. Lung Cell Mol. Physiol. 294 (4), L698-L704 (2008)
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