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RASGRF1 Antibody (S929)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RASGRF1 Antibody (S929) AP7582b
    Western blot analysis of RASGRF1 antibody (S929) (Cat. #AP7582b) in MDA-MB231 cell line lysates (35ug/lane). RASGRF1 (arrow) was detected using the purified Pab.
  • IHC-P - RASGRF1 Antibody (S929) AP7582b
    Formalin-fixed and paraffin-embedded human brain tissue reacted with RASGRF1 Antibody (S929) (Cat.#AP7582b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13972
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 145234 Da
Antigen Region 907-936 aa
Additional Information
Gene ID 5923
Other Names Ras-specific guanine nucleotide-releasing factor 1, Ras-GRF1, Guanine nucleotide-releasing protein, GNRP, Ras-specific nucleotide exchange factor CDC25, RASGRF1, CDC25, GNRP, GRF1
Target/Specificity This RASGRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 907-936 amino acids from human RASGRF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRASGRF1 Antibody (S929) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms CDC25, GNRP, GRF1
Function Promotes the exchange of Ras-bound GDP by GTP.
Research Areas
Citations (0)

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RASGRF1 is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. Studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.


Iguchi,T., Int. J. Oncol. 31 (2), 285-291 (2007)
Holmes,R., Mol. Cell. Biol. 26 (24), 9564-9570 (2006)

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Cat# AP7582b
(40 western blots)
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