|Application ||WB, IHC-P, E|
|Calculated MW||176442 Da|
|Antigen Region||636-666 aa|
|Other Names||ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, ALK|
|Target/Specificity||This ALK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636-666 amino acids from the Central region of human ALK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ALK Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y- x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein Note=Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway|
|Tissue Location||Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells|
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Provided below are standard protocols that you may find useful for product applications.
ALK, a member of the insulin receptor subfamily of Tyr protein kinases, is an orphan receptor. It appears to play an important role in the normal development and function of the nervous system. This Type I membrane protein is expressed in brain and CNS and in the small intestine and testis, but not in normal lymphoid cells. A form of non-Hodgkin's lymphoma is characterized by a chromosomal translocation t(2;5)(p23;q35) that involves NPM1 and ALK. The protein contains 1 LDL-receptor class A domain and 2 putative MAM domains.
Morris, S.W., et al., Oncogene 14(18):2175-2188 (1997).
Iwahara, T., et al., Oncogene 14(4):439-449 (1997).
Morris, S.W., et al., Science 263(5151):1281-1284 (1994).
Morris, S.W., et al., Oncogene 15, 2883-2883 (1997).
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