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EphB4 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EphB4 Antibody AP7625D
    Western blot analysis of EphB4 (arrow) using rabbit polyclonal EphB4 Antibody (Cat.#AP7625d).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB4 gene (Lane 2) (Origene Technologies).
  • IHC-P - EphB4 Antibody AP7625D
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the EphB4 antibody (Cat.#AP7625d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54760
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 108270 Da
Additional Information
Gene ID 2050
Other Names Ephrin type-B receptor 4, Hepatoma transmembrane kinase, Tyrosine-protein kinase TYRO11, EPHB4, HTK, MYK1, TYRO11
Target/Specificity This EphB4 antibody is generated from rabbits immunized with human recombinant EphB4 protein.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEphB4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPHB4
Synonyms HTK, MYK1, TYRO11
Function Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4- mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix
Research Areas
Citations ( 0 )


Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.


Steinle, J.J., et al., J. Biol. Chem. 277(46):43830-43835 (2002). Suenobu, S., et al., Biochem. Biophys. Res. Commun. 293(3):1124-1131 (2002). Wang, Z., et al., Blood 99(8):2740-2747 (2002). Wilson, M.D., et al., Nucleic Acids Res. 29(6):1352-1365 (2001). Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001).

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$ 295.00
$ 99.00
Cat# AP7625D
(40 western blots)
Availability: In Stock
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