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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ERBB3 Antibody (N-term)   

ERBB3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ERBB3  Antibody (N-term) AP7630a
    Western blot analysis of anti-ErbB3 Pab (Cat. #AP7630a) in mouse brain lysate. ErbB3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • IHC-P - ERBB3  Antibody (N-term) AP7630a
    Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ERBB3  Antibody (N-term) AP7630a
    Flow cytometric analysis of 293 cells using ErbB3 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IHC-P - ERBB3  Antibody (N-term) AP7630a
    Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IHC-P - ERBB3  Antibody (N-term) AP7630a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • SPECIFICATION
  • CITATIONS: 4
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P21860
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 148098 Da
Antigen Region 24-55 aa
Additional Information
Gene ID 2065
Other Names Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, ERBB3, HER3
Target/Specificity This ERBB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-55 amino acids from the N-terminal region of human ERBB3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsERBB3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ERBB3
Synonyms HER3
Function Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). May also be activated by CSPG5 (PubMed:15358134).
Cellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein
Tissue Location Epithelial tissues and brain.
Research Areas
Citations ( 0 )

Background

ErbB3, a member of the EGF receptor family, binds and is activated by neuregulins and NTAK. It potentially forms a heterodimer with each of the other ERBB receptors. This protein is predominantly expressed in epithelial tissues and brain. The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. Ligand-binding may increase phosphorylation on tyrosine residues and promote its association with the p85 subunit of phosphatidylinositol 3-kinase ErbB3 is overexpressed in a subset of human mammary tumors.

References

Katoh, M., et al., Biochem. Biophys. Res. Commun. 192(3):1189-1197 (1993). Plowman, G.D., et al., Proc. Natl. Acad. Sci. U.S.A. 87(13):4905-4909 (1990). Kraus, M.H., et al., Proc. Natl. Acad. Sci. U.S.A. 86(23):9193-9197 (1989).

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$ 295.00
$ 99.00
Cat# AP7630a
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(40 western blots)
Availability: In Stock
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